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lipA [2018-01-03 16:29:08]
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lipA [2018-01-03 16:29:08]

trigger enzyme
locus
BSU32330
pI
8.25
mw
33.77 kDa
protein length
298 aa Sequence Blast
gene length
894 bp Sequence Blast
function
lipid metabolism, required for the synthesis of branched-chain amino acids
product
trigger enzyme
essential
no
ec
2.8.1.8
synonyms
yutB
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.7|Biosynthesis and scavenging of lipoic acid]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.3|Trigger enzyme] → [category|SW 3.4.3.5|Trigger enzymes that control transcription in a yet unknown way]

    • Gene

    Coordinates
    3,320,324 → 3,321,220

    Phenotypes of a mutant

  • reduced transformation efficiency [Pubmed|19028902]
  • strong inhibition of growth on minimal media [Pubmed|19820084]

    • The protein

    Catalyzed reaction/ biological activity

  • Protein N(6)-(octanoyl)lysine 2 sulfur 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine 2 L-methionine 2 5'-deoxyadenosine (according to Swiss-Prot) Triacylglycerol H2O = diacylglycerol a carboxylate (according to Swiss-Prot) required for ''[gene|788725411B2E741103603373E43441FD036E1BA0|comEA]'' transcription [Pubmed|19028902]

    • Protein family

  • Lipoyl synthase family (according to Swiss-Prot) AB hydrolase superfamily (according to Swiss-Prot)

    • [SW|Cofactors]

  • Fe-S cluster [pubmed|29292548]

    • Structure

  • [PDB|4U0O] (from ''Thermosynechococcus elongatus'', 48% identity) [Pubmed|25100160]

    • Expression and Regulation

    Operons

    genes
    [gene|2EC820F54911603A99444DC922E41C60AA526EB2|lipA]
    description
    [pubmed|22383849]
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    Biological materials

    Mutant

  • MGNA-B578 (yutB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1577 NBRP B. subtilis, Japan]
  • BKE32330 (Δ[gene|2EC820F54911603A99444DC922E41C60AA526EB2|lipA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE32330 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTCCCATCATCTCCAAT, downstream forward: _UP4_TAATGCCAAAACGCCAGATC
  • BKK32330 (Δ[gene|2EC820F54911603A99444DC922E41C60AA526EB2|lipA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK32330 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTCCCATCATCTCCAAT, downstream forward: _UP4_TAATGCCAAAACGCCAGATC

    • References

    Reviews

  • 27074917

    • Original publications

  • 18957862,19028902,19820084,25100160